中药复方芩藿饮对鸭肝炎病毒实验感染雏鸭的治疗效果分析

doi:10.11843/j.issn.0366-6964.2018.05.018

摘要/Abstract

摘要：

本试验旨在研究中药复方芩藿饮对感染鸭肝炎病毒雏鸭的临床治疗效果。选取300只健康1日龄雏鸭，随机分为5组，每组60只。试验组分别为芩藿饮高剂量组、中剂量组、低剂量组、病毒组、空白组。6日龄时，除空白组外，其他各试验组雏鸭腿部肌肉注射DHAV-1人工染毒造模；雏鸭感染病毒1 h后，芩藿饮高剂量组、中剂量组、低剂量组按照高剂量（0.6 mL·只^-1）、中剂量（0.4 mL·只^-1）、低剂量（0.2 mL·只^-1）添加芩藿饮于饮水中进行治疗，连续给药3 d；病毒组和空白组饮水中不给予任何药物。在给药治疗的第4、8、48小时采集病料测定相关指标。比较各试验组雏鸭的病死率；观察肝病理剖检及HE染色切片；分析雏鸭血液中DHAV-1 VP1基因拷贝数；检测肝功能生化指标（AST、ALT、TP、ALB、GLO）和细胞因子（IL-2、IL-6、IL-8、IFN-β）含量；评价芩藿饮对感染鸭肝炎病毒雏鸭的治疗效果。结果显示，芩藿饮能够有效降低感染雏鸭的病死率；与病毒组相比，芩藿饮能够显著降低感染雏鸭血液中DHAV-1 VP1基因拷贝数（P<0.05）；修复DHAV-1对肝造成的损伤；降低感染雏鸭血清中AST、ALT含量，增加TP、ALB、GLO含量；促进机体IL-2、IL-6、IL-8、IFN-β细胞因子的分泌。高剂量芩藿饮对实验感染性鸭肝炎病毒雏鸭有一定的临床治疗作用。

Abstract:

The purpose of this study was to evaluate the therapeutic effect of traditional Chinese herbal compound of Qinhuo oral liquid on ducklings that infected with duck hepatitis virus. Three hundred feathers healthy 1-day-old ducklings were randomly divided into 5 groups, 60 feathers in each group. The test groups were Qinhuo high dose group, middle dose group, low dose group, virus group and blank control group, respectively. At the age of 6 days old, except for the blank control group, the other groups were intramuscular injected with DHAV-1 to establish artificial infection model; 1 h later, Qinhuo high dose group, middle dose group and low dose group were given high dose (0.6 mL per feather), middle dose (0.4 mL per feather) and low dose (0.2 mL per feather) of Qinhuo oral liquid in the water for treatment, once a day for 3 days, the virus group and blank control group treated without medicine. The samples were collected at the 4th, 8th and 48th hour during the experiment for measuring related indexes. During the experiment, the mortality rate of each group was compared; the liver pathological examination and HE staining section were observed; relative blood DHAV-1 VP1 gene copies number was analyzed; the contents of hepatic function biochemical indexes (AST, ALT, TP, ALB, GLO) and serum cytokines (IL-2, IL-6, IL-8, IFN-β) were detected. All indexes were severed to evaluate the therapeutic effect of Qinhuo oral liquid on duck virus hepatitis. The results showed that Qinhuo oral liquid can effectively reduce the mortality rate of infected ducklings; compared with virus group, Qinhuo oral liquid can significantly reduce the copies number of DHAV-1 VP1 in the blood (P<0.05); repair the liver damage casused by DHAV-1; reduce the contents of AST and ALT; enhance the contents of IL-2, IL-6, IL-8, IFN-β in serum. Considering all factors, the high dose of the Qinhuo oral liquid has a better therapeutic effect on ducklings infected with duck hepatitis virus.

中图分类号:

张伟, 白景英, 杜红旭, 熊文, 明珂, 刘家国. 中药复方芩藿饮对鸭肝炎病毒实验感染雏鸭的治疗效果分析[J]. 畜牧兽医学报, 2018, 49(5): 1027-1036.

ZHANG Wei, BAI Jing-ying, DU Hong-xu, XIONG Wen, MING Ke, LIU Jia-guo. Analysis of the Therapeutic Effect of Traditional Chinese Herbal Compound of Qinhuo Oral Liquid in Ducklings Infected with Duck Hepatitis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 1027-1036.

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